Abstract:

BACKGROUND: Previous studies of animal models of nervous system diseases have shown that bone marrow mesenchymal cells-derived neural stem cells have a good effect on central nervous system diseases.
OBJECTIVE: To observe the therapeutic effects of autologous transplantation of bone marrow mesenchymal cells-derived neural stem cells applied for central nervous system diseases.
METHODS: Seven patients with central nervous system diseases were subjected to by autologous transplantation of bone marrow mesenehymal cells-derived neural stem cells from October 2008 to April 2009. Bone marrow puncture aspiration was used to collected 50-60 mL bone marrow blood, which was prepared into bone marrow mesenchymal stem cell suspension following isolation and culture in vitro. Neural stem cells were isolated and induced with self cerebrospinal fluid, and transplanted into patients by lumbar puncture via the subarachnoid cavity. Neurological impairment and side effects posttransplantation were observed prior to and following transplantation.
RESULTS AND CONCLUSION: No adverse reactions were found in three patients after transplantation. The neurological function of the seven patients had been improved in 6-month follow-up. Results suggest that it is a safe, convenient and effective method to use autologous transplantation of bone marrow mesenchymal cells-derived neural stem cells to treat patients with neurological impairment induced by nervous system diseases.